Hollow stems grow from 2-4 ft. (0.6-1.2 m) tall. Fang, X., Subudhi, P.K., Venuto, B.C. Soc. The Jaccard similarity coefficient computed from RAPD and AFLP data ranged from 22% to 89%, indicating a high level of genetic diversity. Spartina alterniflora is a perennial grass that expands via underground rhizomes. Spartina alterniflorais a rhizomatous grass native to the Atlantic and Gulf coast marshes of North America (Xiao et al. The Jaccard similarity coefficient computed from RAPD and AFLP data ranged from 22% to 89%, indicating a high level of genetic diversity. This study validated the possibility of transforming the abandoned S. alterniflora into a biological resource to inhibit M. aeruginosa blooms through allelopathy. Spartina alterniflora Taxonomía Reino: Plantae División: Fanerógama Magnoliophyta Clase: Liliopsida Orden: Cyperales Familia: Poaceae Subfamilia: Chloridoideae Tribu: Spartina alterniflora (espartillo de cangrejal o borraza) es una gramínea perenne caducifolia que se halla en áreas inundadas intermareales, especialmente estuarios saladares. Goals / Objectives Characterize Spartina alterniflora accessions at the phenotypic and molecular level. (Poaceae, saltmeadow cordgrass), Spartina alterniflora Loisel. Analysis of the selected plants revealed that plants selected from accessions with superior seed germination rates also showed significant differences for all other traits. endstream endobj 97 0 obj <>/Metadata 7 0 R/Pages 94 0 R/StructTreeRoot 14 0 R/Type/Catalog/ViewerPreferences 118 0 R>> endobj 98 0 obj <>/MediaBox[0 0 612 792]/Parent 94 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 99 0 obj <>stream The mean percent germination was 43 % with a range of 3 to 89%. The differences in clustering pattern indicated the potential of molecular markers in accurate assessment of genetic diversity. This plant can be weedy or invasive according to the authoritative sources noted below.This plant may be known by one or more common names in different places, and some are listed above. Spartina has infested Willapa Bay, Washington, and other natural settings and done great damage to the natural marine environment. and Harrison, S.A. 2004. Subudhi, P.K., Parami, N., Ryan, A. and Harrison, S.A. 2003. continuous and long term program with the goal of developing seed propagated synthetic smooth cordgrass varieties which maintain a broader genetic pool.ImpactsCoastal wetland loss continues to be a major environmental problem in the state of Louisiana. DC. Ryan, A.B., Venuto, B., Harrison, S., Subudhi, P., and Materne, M. 2002. Trachynotia alterniflora Steud. [14512] 4. Plant Sci. The average seed set was 49% with a range of 0 to 94%. Spartina alterniflora Loiseleur-Deslongchamps, var. Maintenance of adequate vegetation in the existing and newly restored marshlands can help slow down the coastal wetland loss. To download the original size of this image Using Random Amplified Fragment DNA Polymorphism (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers, 40 genotypes from seven accessions were fingerprinted. Assessment of genetic diversity in Louisiana native smooth cordgrass (Spartina alterniflora) accessions using molecular markers. Large amount of variability was noted for these traits and 45 plants with consistent superior rating were selected for further evaluation. h�bbd```b``� "&�H�� ��̞"Y��9 �� Compared Hybridization between the selected S. alterniflora plants were continued in both greenhouse and field to advance the recurrent selection program. Ecology. Grande Terre, Louisiana, showed that seed set varied greatly both within and among accessions. Proceedings, Southern Branch Amer. The hybrids were evaluated in the marsh, and the best performing ones were identified for next round of crossing and selection. Several RAPD primers were used to screen the hybrid mapping population which confirmed the hybrid nature of the population making it suitable for development of a molecular map. Spartina alterniflora is an invasive grass that has spread to more than 10,000 acres in Washington state and more than 1,000 acres in San Francisco Bay and tidelands. Protocols for fingerprinting Spartina In the southernmost part of coast of China, two height forms of Spartina alterniflora, tall and short, have invaded Leizhou Peninsula within the last decade. 154 0 obj <>stream It is native to the eastern United States, but is considered invasive, in salt marshes, in California. Two field investigations, at Baton Rouge and Burdick, David M. 1989. Eight hundred mutagenized plants were produced from two different mutagens; Ethyl Methane Sulphonate and Sodium Azide, and were evaluated in a marsh location in Progress 01/01/02 to 12/31/02 Outputs Activities included collecting 50 Spartina alterniflora accessions from highly impacted dying marsh sites in Louisiana. Trachynotia alterniflora (Loisel.) Conversely, negative correlations between growth traits and rust reaction were observed. The average seed set at Baton Rouge was 47% and ranged from 0 to 94%. p:12. 165(3):395-401. To determine if Vermilion (a released clone commonly used in restoration projects) seedlings are comparable to The clones are also currently fingerprinted to determine and compare the amount of genetic variation between the clones and seedlings. Invasive Spartina alterniflora (S. alterniflora), a native riparian species in the U.S. Gulf of Mexico, has led to serious degradation to the ecosystem and biodiversity as well as economic losses since it was introduced to China in 1979. Cluster analysis was performed on agronomic and molecular data based on genetic distance within each accession (among plants) and among accessions from the same location. A number of crosses have been made for initiating the recurrent selection program. Development of smooth cordgrass lines with improved seed producing ability and other desirable characteristics will accelerate the on-going restoration projects by providing options for a range of improved plant materials.Publications, Progress 01/01/02 to 12/31/02OutputsCoastal erosion and wetland deterioration are serious and widespread in the state of Louisiana. (smooth cordgrass) (Denno 1977, 1978), with Spartina alterniflora “…an inferior host plant for development” (Denno 1977: 366). glabra (Muhlenberg ex Elliott) Fernald, Rhodora 18: 178. 117 0 obj <>/Filter/FlateDecode/ID[<5AA68FA4C9FF314BB0D91E1A72901282>]/Index[96 59]/Info 95 0 R/Length 108/Prev 148061/Root 97 0 R/Size 155/Type/XRef/W[1 3 1]>>stream Above- and belowground biomass and nutrient pools of Spartina alterniflora (smooth cordgrass) in a South American salt marsh Author: Negrin, Vanesa L., de Villalobos, Ana E., Trilla, Gabriela González, Botté, Sandra E., Marcovecchio, Jorge E. Click on an acronym to view each weed list, or click here for a composite list of Weeds of the U.S. common cordgrass Spartina anglica C.E. 2003). S. alterniflorahas been introduced to new regions both intentionally and unintentionally. 0 The mean percent germination was 43 % with a range of 3 to 89%. The hybrids will be evaluated in the marsh condition, and the best performing ones will be identified for next round of crossing and selection. The differences observed in clustering pattern in both studies indicated that the use of molecular markers was an effective means to assess genetic diversity. Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. A population of 100 hybrids involving two smooth cord grass lines has been generated for construction of a genetic map to locate the quantitative trait loci for seed characteristics. Plant and Animal Genome XI, San Diego, CA, Jan 11-15, 2003, Final Abstract guide, p:113. Positive correlations were found among all vegetative traits representing plant growth. Spartina brasiliensis Raddi Spartina glabra Muhl. Fusarium palustre was associated with the dieback of Spartina alterniflora in its native North American saltmarshes. A total of 40 plants selected from nine accessions were subjected to both field evaluation and DNA fingerprinting using RAPD and AFLP markers. Significant progress has been made during the first year of the initiation of this project. to the original 126 native accessions, the selected plants demonstrated a significant improvement in many of the seed and germination related traits. This grass can be found growing on sandy aerobic or anaerobic soils with pHs ranging from 3.7 to 7.9. We isolated a v-ATPase gene (SaVHAc1) from Spartina alterniflora or smooth cordgrass, a plant native to Louisiana coastal salt marshes and expressed it in transgenic tobacco and rice. Spartina alterniflora Loisel. Smooth cordgrass will tolerate regular inundations with 0 to 35 parts per thousand salinity. AFLP analysis of another set of 38 Compared to the original 126 native accessions, these results demonstrated a significant improvement. h�b```a``�"e�@����(����3 � (����� �+�� -�"`�?Y�2�a^���\�����Μq/E���J:Q�I����lL��4#��fN&{)�(S@� ~�n [15194] 3. Spartina alterniflora) in June and the results in October USDA is an equal opportunity provider, employer, and lender. Spartina alterniflora. Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. In case of RAPD, 176 fragments were polymorphic where as 708 fragments were polymorphic in AFLP analysis. Ryan, A.B., Subudhi, P.K., Venuto, B.C., Harrison, S.A., and Shadow, R.A. 2003. An automated system was developed and used to generate regular and random Data Source and References for Spartina alterniflora (smooth cordgrass) from the USDA PLANTS database Name Search name search type enter a search name }Pc��؆�nͿ�%ٲl�J��� �����s�;�gBx&5�3���+l$@��)�L(��f�ѸAn��2+���4�-.�i@��4S |��\R{�B��ăxG$ͤpD2L*�a�KjK�����p�PG0�h:H�4�(���).��Ng ��0����c�$��;���\^hT�Si���L���b����z9K��q^�2V��fo�"��&��X=��h��̢�oŻQ�Yt�2��. Ecology. A molecular genetics laboratory has been established within the LSU Agricultural Center's Department of Agronomy to conduct research involving wetland plant species. Preliminary results show considerable natural variation among the original collections across south Louisiana for traits contributing to plant establishment from seed. Phytopathology 93:S74 (Abstr.). Late flowering accessions were associated with increased spread and tiller density but not with the other traits. Ryan, A.B., Subudhi, P.K., Venuto, B.C., Harrison, S.A. and Shadow, R.A. 2003. Spartina alterniflora Loisel. In case of RAPD, 176 fragments were polymorphic where as 708 fragments were polymorphic in AFLP analysis. A number of crosses were made each year in the recurrent selection program. Progress 09/01/01 to 03/31/04OutputsThe overall goal of this project is to apply plant breeding and genetics principles to develop improved smooth cordgrass (Spartina alterniflora) lines for utilization in coastal restoration projects. %PDF-1.7 %���� Mode of pollination, pollen germination, and seed set in smooth cordgrass (Spartina alterniflora, Poaceae). Phytopathology 93:S74 (Abstr.). Nevertheless, since all sampled individuals represent unique genotypes and all sampled marshes exhibit relatively - smooth cordgrass SPAL Provided by USDA NRCS Golden Meadows PMC (LAPMC). Usage Requirements. A population of 100 hybrids involving two smooth cord grass lines has been generated for construction of a genetic map to locate the QTL for seed characteristics. DNA fingerprinting protocols developed through this project will help to test the authenticity of plant materials used in the restoration projects. Spartina glabra Muhl. 1916. Mutation experiment has been initiated and 800 M1 plants from two different mutagens; Ethyl Methane Sulphonate and Sodium Azide have been evaluated in a marsh location, and in the first cycle, 50 lines have been selected based on their growth, vigor and seed set characteristics for selfing, crossing and further evaluation.ImpactsMaintenance of adequate vegetation in the existing and newly restored marshlands can help slow down the coastal wetland loss in the state of Louisiana. Large amount of variability was noted for these traits and 45 plants with consistent superior growth characteristics were selected for further evaluation. Spartina alterniflora: Preserving, protecting and defending coastal Louisiana. Rescuing the coast with biotechnology. Characterization of Louisiana native Spartina alterniflora populations. Assessment of genetic diversity in Louisiana native smooth cordgrass (Spartina alterniflora) accessions using molecular markers. Positive correlations were found among all vegetative traits representing plant growth. Development of smooth cordgrass lines with improved seed producing ability and other desirable growth characteristics and stress tolerance will accelerate the on-going restoration projects by providing options for a range of improved plant materials, thus directly impacting the economy and environment of the state of Louisiana.Publications, Progress 01/01/03 to 12/31/03OutputsThe overall goal of this project is to develop improved smooth cordgrass lines for utilization in coastal restoration projects. Spartina alterniflora, the smooth cordgrass, saltmarsh cordgrass, or salt-water cordgrass, is a perennial deciduous grass which is found in intertidal wetlands, especially estuarine salt marshes. 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